吕湘课题组简介

吕湘，女，研究员，博士生导师，中国生理学会血液学专业委员会青年委员，中国研究型医院学会血液病精准诊疗专业委员会委员，协和学者特聘教授。

1991年-1995年山东大学生物系，获理学学士学位；1995-1998年就读于中国科学院发育生物学研究所，获硕士学位；1999-2002年中国协和医科大学博士研究生，获得生化与分子生物学专业博士学位；毕业后留所工作，历任助理研究员、副研究员；2004.11-2006.7 受法中科学基金（FFCSA）资助在法兰西学院（INSERM U36）进行博士后研究；2013.7任生化系研究员。2015.10 聘任为病理生理系课题组长。承担和参加了多项自然科学基金、863、973、国家重点研发专项等国家级科研课题及院校创新工程项目。近年工作主要从3D基因组组织及基因长距离调控层面研究造血分化，发现核基质结合蛋白SATB1、SATB2、CTCF、核体蛋白PML4等因子及非编码RNA分子在人β-珠蛋白基因、HOXA基因等造血相关基因簇染色质高级构象组织和发育阶段性表达中的重要调控功能。主要研究工作发表在Blood, Gene & Dev, J Hepat, Cell Res, Nucleic Acids Res, Oncotarget, Mol Cell Biol, J Biol Chem，Sci Rep-UK等杂志。共发表SCI论文35篇，核心期刊论文6篇，其中作为第一作者和通讯作者的论文15篇。2002年获中华医学科技奖一等奖。

研究方向
从事造血分化的多层次基因表达调控研究，近年主要关注染色质高级构象层面的转录调控机制，包括：
1. 正常造血分化和疾病状态下的3D基因组动态组织及其功能研究；
2. 造血关键转录因子基因座的局部染色质高级构象组织和长距离调控研究；
3. 通过靶向关键基因的顺式调节元件改造和提高体外造血分化效率。

课题组人员：

助理研究员：刘雪会博士
技术员：韩改净
直博生：杨科
硕士研究生：史丽芳、韩玲
已毕业硕士研究生：崔申申

代表性研究论文：
1. Dan-Dan Zhang*, Wen-Tian Wang*, Jian Xiong*, Xue-Min Xie, Shen-Shen Cui, Zhi-Guo Zhao, Mulin Jun Li, Zhu-Qin Zhang, De-Long Hao, Xiang Zhao, Yong-Jun Li, Jun-Wen Wang, Hou-Zao Chen, Xiang Lv#, De-pei Liu#. Long noncoding RNA LINC00305 promotes inflammation by activating the AHRR-NF-κB pathway in human monocytes. Sci Rep. 2017,7:46204. (#co-corresponding author)
2. Xi-Zhou An*, Zhi-Guo Zhao*, Yu-Xuan Luo, Ran Zhang, Xiao-Qiang Tang, DeLong Hao, Xiang Zhao, Xiang Lv#, De-Pei Liu#. Netrin-1 suppresses the MEK/ERK pathway and ITGB4 in pancreatic cancer. Oncotarget, 2016, 7(17):24719-24733 (#co-corresponding author)

3. Guo-You Liu*, Guang-Nian Zhao*, Xiao-Feng Chen, De-Long Hao, Xiang Zhao, Xiang Lv#, De-pei Liu#. The long noncoding RNA Gm15055 represses Hoxa gene expression by recruiting PRC2 to the gene cluster. Nucleic Acids Res, 2016, 44(6):2613-27 (#co-corresponding author)

4. Miao Xu*, Guan-gnian Zhao*, Xiang Lv*, Guo-you Liu, Lily Yan Wang, De-Long Hao, Jun-wen Wang, De-Pei Liu#, Chih-Chuan Liang. CTCF controls HOXA cluster silencing and mediates PRC2-repressive higher-order chromatin structure in NT2/D1 cells. Mol Cell Biol, 2014, Oct 15, 34(20):3867-79. (*co-first author)
5. Jie Wu*, Li-Quan Zhou*, Wei Yu*, Zhi-Guo Zhao, Xue-Min Xie, Wen-Tian Wang, Jian Xiong, Man Li, Zheng Xue, Xing Wang, Peng Zhang, Bei-Bei Mao, De-Long Hao, Xiang Lv#, De-Pei Liu1#. PML4 facilitates erythroid differentiation by enhancing the transcriptional activity of GATA-1. Blood, 2014 Jan 9; 123(2):261-70. (#co-corresponding author)
6. Li-Quan Zhou*, Jie Wu*, , Wen-Tian Wang, Guang-Nian Zhao, Wei Yu, Peng Zhang, Jian Xiong, Man Li, Zheng Xue, Xing Wang, Zhi-Chen Guo, Xiang Lv#, De-Pei Liu#. The AT-rich DNA-binding protein SATB2 promotes expression and physical association of human Gγ- and Aγ-globin genes. J Biol Chem 2012, Aug 31 287(36):30641-52 (# co-corresponding author).
7. Zheng Xue*, Xiang Lv*, Wei Song*, Xing Wang, Guang-Nian Zhao, Wen-Tian Wang,Jian Xiong, Bei-Bei Mao, Wei Yu, Ben Yang, Jie Wu, Li-Quan Zhou, De-Long Hao,Wen-Ji Dong, De-Pei Liu* and Chih-Chuan Liang. SIRT1 deacetylates SATB1 to facilitate MARHS2- MARε interaction and promote ε-globin expression. Nucleic Acids Research, 2012, Jun 1, 40(11):4804-15 (*co-first author)
8. Beibei Mao, Guowei Zhao, Xiang Lv#, Hou-Zao Chen, Zheng Xue, Ben Yang, De-Pei Liu#, Chih-Chuan Liang. Sirt1 deacetylates c-Myc and promotes c-Myc/Max association. Int J Biochem Cell Biol, 2011, 43(11):1573-1581. (#co-corresponding author)
 9. Lu Lu, Lei Li, Xiang Lv, Xue-Song Wu, De-Pei Liu, Chih-Chuan Liang. Modulations of hMOF autoacetylation by SIRT1 regulate hMOF recruitment and activities on the chromatin. Cell Res 2011:1-14
10. Zhen-Ya Li, Yang Xi, Wen-Nan Zhu, Chao-Zeng, Zhu-Qin Zhang, Zhi-Chen Guo, De-Long Hao, Guang Liu, Lei Feng, Hou-Zao Chen, Feng Chen, Xiang Lv, De-Pei Liu, Chih-Chuan Liang. Positive Regulation of Hepatic miR-122 Expression by HNF4α.J Hepatol. 2011 Sep; 55(3):602-11.
11. Bruno Larrivée, Catarina Freitas, Marc Trombe, Xiang Lv, Benjamin DeLafarge, Li Yuan, Karine Bouvrée1, Christiane Bréant, Françoise Bono, Filip Claes, Monica Autiero, Peter armeliet, Marc Tessier-Lavigne and Anne Eichmann. Activation of the UNC5B receptor by Netrin-1 inhibits neovascularization. Gene Dev. 2007, 21: 2433-2447.

国际会议特邀报告

1. ‘Gene regulation at three-dimension’, 2014 annual meeting of FONDATION FRANCO-CHINOISE POUR LA SCIENCE ET SES APPLICATIONS (FFCSA), 2014 Jan.10-12, Harbin,China.
2. ‘Gene regulation at three-dimension: progresses in globin gene cluster study’, International Symposium on Erythrocyte Biology, 2014 Apr.24-25, ZhengZhou, China.
3. ‘Spatial Control of Globin Gene Expression’ ,3rd International Conference on Thalassemia in China & 2nd Asia-Pacific Iron Academy Conference, 2010 Nov.3-6, Nanning, China.
4. ‘Wiring to control---regulating clustered genes in three-dimensional chromatin organization’, International Conference of Genomics, 2009 Oct.21-23, Beijing, China.

获奖情况
1. 2002 年参与完成的“建立α与β珠蛋白基因簇转基因鼠模型及β基因簇反式因子的研究”获中华医学科技奖一等奖
2.  Young Scientist travel fellowship for the XIX International Congress of Biochemistry and Molecular Biology held jointly with the 2nd Annual Congress of the HUman Proteome Organization (2003)
3.  Fellowship from Fondation Franco-Chinois pour Science et ses Applications (FFCSA) (Nov. 2004-Nov. 2005)

4.  Fellowship from Fondation Treilles (Mar. 2006)
5.  European commission Marie Curie Actions Scholarship (European School of Haematology) (May 2006)